Xypex Chemical Corporation -Bio-San® C500 Antimicrobial Concrete Additive


Xypex Bio-San C500 is a uniquely designed admixture for integral, long-term protection of concrete in harsh sewage conditions with high levels of H2S that cause microbial induced corrosion.

Category: antimicrobial addmixtures | cementitious waterproofing and dampproofing | crystalline waterproofing...

MasterFormat: Common Work Results for Concrete | Cast-In-Place Concrete | Dampproofing and Waterproofing...

Xypex Chemical Corporation
13731 Mayfield Place
Richmond, BC V6V 2G9
Tel: (800) 961-4477



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Bio-San® C500 Antimicrobial Concrete Additive

Xypex Bio-San C500 is a uniquely designed admixture for integral, long-term protection of concrete in harsh sewage conditions with high levels of H2S that cause microbial induced corrosion. Bio-San C500 combines potent antimicrobial protection along with the unique crystalline technology of the Xypex Admix C-Series.

Bio-San contains bio-active mineral solids that become permanently fixed within the cement matrix impairing bio-film formation thus inhibiting the growth of acid causing sewer bacteria such as Thiobacillus due to high concentrations of H2S. The unique Xypex crystalline technology creates a permanent structure throughout the pores and capillary tracts providing waterproofing and enhanced chemical protection including acid and sulphate resistance. Bio-San C500 prevents microbial induced corrosion, stops infiltration/exfiltration of water, and provides acid and sulphate resistance, significantly extending the service life of concrete sewage collection systems and waste water infrastructure.

Recommended for:
  • Manholes / Sewer Pipes
  • Pump and Lift Stations
  • Head Works
  • Septic Tanks
  • Digesters
  • Clarifiers
  • Industrial Structures
  • Inhibits microbial induced corrosion
  • Resists extreme hydrostatic pressure
  • Resistant to aggressive chemicals (acids and sulphates)
  • Can seal static hairline cracks up to 0.4 mm
  • Becomes a permanent, integral part of the substrate and cannot be punctured, damaged or lose adhesion
  • Does not contain any VOCs
  • Less costly to apply than most other methods
  • Added to the concrete at the time of batching and therefore not subject to weather and surface moisture constraints

• Long retention sewer lines
• Manholes
• Headworks and lift stations
• Drop structures
• Force main outflows
• Enclosed sewage plant structures

For maximum protection of concrete in severe sewage conditions.

Xypex Bio-San C500 is a unique way of protecting concrete in harsh sewage conditions with high levels of hydrogen sulphide that cause microbial induced corrosion. No other admixture combines potent antimicrobial properties with Xypex crystalline waterproofing technology to offer complete protection of concrete sewer and wastewater structures.

Xypex Bio-San C500 contains mineral solids that remain permanently fixed within the concrete throughout the life of the sewage structure. These kill acid-generating microbes such as Thiobacillus that proliferate in high H2S conditions thus preventing microbial induced corrosion (MIC). Combined with Xypex’s proven crystalline technology, Bio-San C500 will protect concrete from infiltration and exfiltration as well as resisting acid and sulphate attack. After placement and curing, the crack-healing properties of Xypex will continue to function throughout the service life of the concrete.

As with all Xypex Admix products, Bio-San C500 is added at the time of concrete batching, avoiding the potential quality issues of liners or coatings and eliminating schedule delays for surface preparation. As a one-component product, Bio-San C500 simplifies the production process for pre-cast structures.

Xypex Bio-San C500 is recommended for use in sewage systems especially in areas where H2S gas is likely to accumulate. This includes sewer lines with long retention times (flat, long, low flow) and sealed or unvented manholes. It will also include areas of high turbulence such as lift stations, drop structures, force main outflows and head works as well as various areas within wastewater treatment plants.

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Proven Technology
Kills the acid-generating microbes present in sewage environments

Independent testing showed that in concrete treated with Xypex Bio-San C500 the presence of the sewer bacteria Thiobacillus Novellus (Starkeya Novella) was substantially reduced.

Protects concrete in high concentrations of H2S

Specimens of concrete treated with Xypex Bio-San as well as untreated concrete were hung in the enclosed sedimentation tanks at a wastewater facility with H2S concentrations of over 50 ppm. The photos of the untreated concrete show the extent of the microbial induced corrosion after 6.5 years. After 10 years the concrete treated with Xypex Bio-San had 9 times less corrosion than the untreated concrete, and continued to function at full efficacy.

Contains Crystalline Technology

Xypex Bio-San C500 contains the same unique crystalline technology as all Xypex Admix products. Xypex treated concrete resists extreme hydrostatic pressure, protects concrete against aggressive chemicals such as acids or sulphates and self-heals static cracks up to 0.4 mm.

In a site test, Xypex treated and untreated samples were analyzed using Scanning Electron Microscopy. Xypex treated samples showed conclusive evidence of the dense crystalline matrix typical of Xypex Crystalline Technology.

Self-heals cracks up to 0.4mm

Concrete panels were cast and then cracked by force after which they were subjected to the ponding of water to measure the flow through the crack. After four days the 0.38 mm crack on the Xypex treated sample had stopped leaking completely. The 0.25 mm crack in the control sample continued to leak after 25 days.

Protects concrete in acidic conditions

Cement mortar samples containing Xypex were subjected to 5% sulphuric acid for up to 12 weeks. Compared to untreated control samples, the weight loss caused by acid attack was reduced by 48%. In a further test on a 30% fly-ash mortar, the weight loss was reduced to 53%.

Protects concrete in sulphate environments

Samples of both Xypex treated and untreated concrete were placed in a highly concentrated sulphate solution. After 4 months the untreated samples experienced a weight loss of 4,800 g/m2whereas the Xypex treated samples only lost between 5 g/m2 and 50 g/m2, showing no visible signs of surface deterioration.

Prevents infiltration & exfiltration of liquids even under extreme hydrostatic pressure

When subjected to 107 m (350 ft) of hydrostatic pressure Xypex treated samples completely resisted the flow of water and only allowed water penetration of 1.5 mm (0.06 in). Conversely, untreated control samples started leaking within 24 hours.